#PATHER WEB MOD#

(optional but HIGHLY RECOMMENDED) Add a custom REFERENCE LIST and re-run the analysis. These results are based on enrichment relative the set of all protein-coding genes in the genome you selected in step 3.Ħ. You will be redirected to the results on the PANTHER website. Note that you will be able to upload a REFERENCE (aka “background”) LIST at a later step.ĥ. Select the species your genes come from (Homo sapiens is default).Ĥ. Select the GO aspect (molecular function, biological process, cellular component) for your analysis (biological process is default).ģ.

#PATHER WEB MOD#

The tool can handle both MOD specific gene names and UniProt IDs (e.g. Paste or type the names of the genes to be analyzed, one per row or separated by a comma.

The list of supported gene IDs is available from the PANTHER website. The PANTHER classification system is explained in great detail in Mi H et al, PMID: 23868073. This service connects to the analysis tool from the PANTHER Classification System, which is maintained up to date with GO annotations. Users can perform enrichment analyses directly from the home page of the GOC website.

For example, given a set of genes that are up-regulated under certain conditions, an enrichment analysis will find which GO terms are over-represented (or under-represented) using annotations for that gene set.

One of the main uses of the GO is to perform enrichment analysis on gene sets.

Pather web